Eukaryotic DNA Polymerases in Homologous Recombination

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Eukaryotic DNA Polymerases in Homologous Recombination.

Homologous recombination (HR) is a central process to ensure genomic stability in somatic cells and during meiosis. HR-associated DNA synthesis determines in large part the fidelity of the process. A number of recent studies have demonstrated that DNA synthesis during HR is conservative, less processive, and more mutagenic than replicative DNA synthesis. In this review, we describe mechanistic ...

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Eukaryotic DNA Polymerases

Themajor function ofDNApolymerases is to replicate the genome and thus to allow transmission of genetic information fromone generation to the next. In eukaryotic cells, this takes place during a discrete period (S phase) of interphase, and replicated chromosomes are subsequently segregated to daughter cells during mitosis. As well as replicating DNA, polymerases help to maintain the integrity o...

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Eukaryotic DNA polymerases.

Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during cell division, DNA repair, DNA recombination, and the by...

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Mechanism of eukaryotic homologous recombination.

Homologous recombination (HR) serves to eliminate deleterious lesions, such as double-stranded breaks and interstrand crosslinks, from chromosomes. HR is also critical for the preservation of replication forks, for telomere maintenance, and chromosome segregation in meiosis I. As such, HR is indispensable for the maintenance of genome integrity and the avoidance of cancers in humans. The HR rea...

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Biochemistry of eukaryotic homologous recombination.

The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased c...

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ژورنال

عنوان ژورنال: Annual Review of Genetics

سال: 2016

ISSN: 0066-4197,1545-2948

DOI: 10.1146/annurev-genet-120215-035243